![]() Therefore, it is important to search out new and potent antimicrobial agents to overcome the problems raised by various resistant pathogenic organisms by scouting untapped resources (Xing et al. This problem has risen due to the certain factors, such as over and misuse of antibiotics, poor hygienic conditions, increased number of patients with weakened immune system and delay in diagnosis of diseases (Bockstael and Aerschot 2008). The emergence and spread of antimicrobial resistance by pathogenic microorganisms to commercial drugs is a noteworthy issue endured by mankind and has turned into an important concern throughout the globe (Rajeswari et al. The clinical relevance of the study was justified as it showed good antimicrobial efficacy against some resistant clinical isolates, too. The chloroformic extract exhibited the good antibiofilm potential and was also found to be biosafe. The efficacy of the extract was also established in terms of post antibiotic effect which ranged from 2 to 20 h. The chloroformic extract showed minimum inhibitory concentration ranging from 0.05 to 5 mg/ml and its microbicidal nature was established by viable cell count studies. Chloroform was found to be the best extractant. The statistical optimization resulted in up to 1.33 fold increase in antimicrobial activity. In the classical optimization yeast peptone dextrose medium, inoculum size of two discs, incubation period of 6 days, production temperature of 25 ✬ and pH 7 was best supportive for optimal growth and antimicrobial activity whereas maltose and ammonium nitrate were the best carbon and nitrogen sources, respectively. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial potential and is optimized for physiochemical parameters to enhance the antimicrobial metabolites production. ![]() ![]() The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. ![]()
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